Ka whakamahia tenei kete mo te in vitro qualitative acid nucleic detection ofMonkeypox Virus i roto i te serum Tangata, nga tauira exudate lesion metauira paku.Ko nga huinga tuatahi me nga tohu tohu FAM kua hangaiamo te kitenga motuhake o Monkeypox Virus, Human RNase P iraka tangohia tahi me te tauira whakamatautau e whakarato ana i te rotomana ki te whakamana i te tukanga tangohanga karihi me te reagenttapatahi.Ko te rangahau e aro ana ki te ira RNase P tangata kua tapaina ki te VIC.
| Waehanga | 48 Nga whakamatautau/kete | 96 Nga whakamatautau/kete |
| He parepare tauhohenga PCR | 672 μL × 1 ngongo | 672 μL × 2 ngongo |
| PCR ranunga whākōkī | 50 μL × 1 ngongo | 100 μL × 1 ngongo |
| Te whakahaere pai | 100 μL × 1 ngongo | 200 μL × 1 ngongo |
| Mana kino | 100 μL × 1 ngongo | 200 μL × 1 ngongo |
1. Te Maamaa: 200 kape/mL.
2. Te Taapiri: Karekau he tauhohenga whakawhiti me te Enterovirus(EV), te karawakahuaketo(MV), Rubella huaketo(RV), Varicella-zoster huaketo(VZV),Dengue huaketo(DenV), TangataParvovirus B19(HPVB19),huaketo Epstein-barr (EBv), huaketo herpes tangata 6(HHV-6)
3. Tika: CV ≤ 5%.
Pūnaha PCR Wā-tūturu: Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad/CFX96 Touch ，Hongshi SLAN-96S/96P，AGS8830, AGS4800