Ka whakamahia tenei kete mo te rapunga waikawa karihi in vitro mo te Monkeypox Virus me te putunga o Awherika ki te uru/te uru i roto i te serum Tangata, nga tauira exudate lesion me nga tauira paku.Ko nga huinga tuatahi me nga rangahau tapanga FAM he mea hoahoa mo te rapunga motuhake mo te Monkeypox Virus, ROX tapanga tirotiro mo te putunga o Awherika, CY5 tapanga rangahau mo te uru o Awherika ki te hauauru.Ko te ira RNase P Tangata i tangohia i te wa kotahi me te tauira whakamatautau e whakarato ana i te mana o roto hei whakamana i te tikanga tangohanga karihi me te pono o te reagent.Ko te rangahau e aro ana ki te ira RNase P tangata kua tapaina ki te VIC.
| Waehanga | 48 Nga whakamatautau/kete | 96 Nga whakamatautau/kete |
| He parepare tauhohenga PCR | 672 μL × 1 ngongo | 672 μL × 2 ngongo |
| PCR ranunga whākōkī | 50 μL × 1 ngongo | 100 μL × 1 ngongo |
| Te whakahaere pai | 100 μL × 1 ngongo | 200 μL × 1 ngongo |
| Mana kino | 100 μL × 1 ngongo | 200 μL × 1 ngongo |
1. Te Maamaa: 200 kape/mL.
2. Tauwhāititanga: Karekau he tauhohenga whakawhiti me te Enterovirus(EV), te mate karaehe (MV), te mate Rubella (RV), te huaketo Varicella-zoster (VZV), te huaketo Dengue (DenV), te Parvovirus tangata B19 (HPVB19), te huaketo Epstein-barr (EBv), huaketo herpes tangata 6(HHV-6)
3. Tika: CV ≤ 5%.
Pūnaha PCR Wā-tūturu:Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad/CFX96 Touch ，Hongshi SLAN -96S/96P，AGS8830, AGS4800