Ka whakamahia tenei kete mo te in vitro qualitative acid nucleic detection of 2019-Novel Coronavirus (2019-nCoV), Rewharewha A me te Rewharewha B i te wa kotahi i roto i nga tauira manawa, tae atu ki te oropharyngeal swabs me te sputum.Ko nga huinga tuatahi me nga rangahau tapanga FAM he mea hoahoa mo te rapunga motuhake o nga ira ORFlab me te N o 2019-nCoV, te tirotiro tapanga VIC mo te ira RNase P tangata hei mana o roto, te tirotiro tapanga ROX mo te ira M o te rewharewha A, te tirotiro tapanga CY5 mo te ira NS o Rewharewha B.
Waehanga | 50 nga whakamatautau / kete |
RT-PCR parepare tauhohenga | 915 μL ×1 ngongo |
RT-PCR ranunga whākōkī | 75 μL ×1 ngongo |
Te whakahaere pai | 100 μL × 1 ngongo |
Mana kino | 100 μL × 1 ngongo |
Tairongo: 200 kape/mL.
Tauwhāititanga:Tautanga:Karekau he tauhohenga whakawhiti ki te SARS-CoV, MERS-CoV, CoV-HKU1, CoV-OC43, CoV-229E, CoV-NL63, Parainfluenza Virus, Human Adenovirus, Tangata Rhinovirus, Respiratory Syncytial Virus (A), Respiratory Syncytial Virus (B), Enterovirus A, Staphylococcus Aureus, Streptococcus Pneumonia, Klebsiella Pneumonia.
Tikanga: CV ≤ 5%.
Pūnaha PCR Wā-tūturu:Aehealth Diagenex AL,Molarray MA-6000, ABI 7500, ViiATM7, QuantStudio 7 flex, Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/Q, Bio-Rad CFX96 PaTM/iQTM5,SLAN-96S/96P