64T | 96T | ||
Waehanga | Inenga | Waehanga | Inenga |
Pereti reagent | 4 | Lysis buffer B | 2 |
Lysis buffer B | 1 | Pereti Lysis | 1 |
ringaringa kirihou | 8 | Horoi 1 pereti | 1 |
pukapuka kawa | 1 | Horoi 2 pereti | 1 |
|
| He pereti elution | 1 |
|
| ringaringa kirihou | 1 |
|
| pukapuka kawa | 1 |
96-poka Porowhita Te Whakarite Pereti
Ko nga waahanga 64T ki te pereti pai e whai ake nei:
Pae pai | 10r7 | 2or8 | 3o9 | 4or10 | 5orll | 6orl2 |
Kete Waehanga | Raihi Kaipupuri 600μL | Horoi Kaipupuri1 500μL | Horoi Pūreirei2 500μL | Pātea | aukume He pirepire 310μL | Elute Kaipupuri l00μL |
Fkete 64T ranei:
Ata tango i te kiriata hiri wera i runga i te pereti reagent, katahi ka tapirihia te 200μL o te tauira me te 20μL o te lysis buffer B ki te pou 1/7 o te pereti reagent.
Mo te kete 96T:
Ata tango i te kiriata hiri wera i runga i te pereti reagent, katahi ka tapirihia te 200μL o te tauira me te 20μL o te lysis buffer B ki te pereti lysis.
Whakauruhia te pereti reagent me te ringaringa kirihou ki roto i te waahi kua tohua o te taputapu i runga i te raupapa, ka paato ki te whakahaere i te kaupapa tangohanga "DNA/RNA" i runga i te tangohanga waikawa karihi.
I te mutunga o te kaupapa, tangohia te ringaringa kirihou ka maka.
Tangohia te pereti elution, ka tangohia te eluent ka rongoa ki roto i te ngongo centrifuge hou mo nga whakamatautau whakararo.Mena kaore e taea te whakahaere i te whakamatautau o raro i te waa, ka taea te penapena i te tauira DNA ki te -20 ℃ ka taea te penapena i te tauira RNA ki te -80 ℃.